Green tissue-specific analysis of a cloned rbcS promoter from Lemna gibba

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Green Tissue-Specific Analysis of a Cloned rbcS Promoter from Lemna gibba

Wang Y. (2014): Green tissue-specific analysis of a cloned rbcS promoter from Lemna gibba. Czech J. Genet. Plant Breed., 50: 235–240. Many plant genetic engineering taskss require the spatial expression of genes which in turn depends upon the availability of specific promoters. The present paper analyses the green-tissue characteristics of a new L. gibba rbcS promoter driving the expression of ...

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Post-transcriptional regulation of organ-specific expression of individual rbcS mRNAs in Lemna gibba.

Many studies of nuclear genes encoding chloroplast proteins have focused on the transcriptional regulation of their expression. The genes (rbcS) encoding the small subunit (SSU) of ribulose-1,5-bisphosphate carboxylase/oxygenase, a major stromal protein, comprise one such group. We have examined the role played by post-transcriptional events in determining the relative levels of individual rbcS...

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A light-regulated DNA-binding activity interacts with a conserved region of a Lemna gibba rbcS promoter.

We have characterized a DNA-binding activity, designated light-regulated nuclear factor (LRF-1), which interacted with a specific sequence located 150 nucleotides upstream from the transcription start site of a phytochrome-regulated Lemna gibba rbcS gene (SSU5B). There was a higher level of LRF-1 activity recovered from nuclei of light-grown plants than from dark-treated plants. In light-grown ...

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A Light - Regulated DNA - Binding Activity lnteracts with a Conserved Region of a Lemna gibba rbcS Promoter Jeffrey

We have characterized a DNA-binding activity, designated light-regulated nuclear factor (LRF-l), which interacted with a specific sequence located 150 nucleotides upstream from the transcription start site of a phytochromeregulated Lemna gibba rbcS gene (SSUSS). There was a higher level of LRF-1 activity recovered from nuclei of light-grown plants than from dark-treated plants. In light-grown p...

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Two 10-bp regions are critical for phytochrome regulation of a Lemna gibba Lhcb gene promoter.

Two small regions of the promoter of an Lhcb gene encoding a light-harvesting chlorophyll a/b protein were identified as essential in conferring phytochrome responsiveness by using a transient expression assay. Initially, 5' deletion analysis of cabAB19, an Lhcb2 gene of Lemna, showed that sequences within the region from -174 to -104 relative to the start of transcription were necessary for ph...

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ژورنال

عنوان ژورنال: Czech Journal of Genetics and Plant Breeding

سال: 2014

ISSN: 1212-1975,1805-9325

DOI: 10.17221/200/2013-cjgpb